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lrg2 1 gfp sgrna vector  (Addgene inc)


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    Structured Review

    Addgene inc lrg2 1 gfp sgrna vector
    KEY RESOURCES TABLE
    Lrg2 1 Gfp Sgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lrg2+1+sgrna+gfp+vectors/LRG2%2E1+(Plasmid+%23108098)/pmc10961051-763-24-27
    Average 94 stars, based on 83 article reviews
    lrg2 1 gfp sgrna vector - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "A membrane-associated MHC-I inhibitory axis for cancer immune evasion"

    Article Title: A membrane-associated MHC-I inhibitory axis for cancer immune evasion

    Journal: Cell

    doi: 10.1016/j.cell.2023.07.016

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Control, Purification, Blocking Assay, Virus, Recombinant, Protease Inhibitor, Transfection, Immunoprecipitation, Activation Assay, Magnetic Beads, Reverse Transcription, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Double Knockout, shRNA, Real-time Polymerase Chain Reaction, Genome Wide, Plasmid Preparation, Software, Flow Cytometry

    Related Articles

    In Vivo:

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. In vivo transplantation of MOLM-13 cells into NSG mice MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Transplantation Assay:

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. In vivo transplantation of MOLM-13 cells into NSG mice MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Transduction:

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. In vivo transplantation of MOLM-13 cells into NSG mice MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Luciferase:

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. In vivo transplantation of MOLM-13 cells into NSG mice MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Expressing:

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. In vivo transplantation of MOLM-13 cells into NSG mice MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Plasmid Preparation:

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. In vivo transplantation of MOLM-13 cells into NSG mice MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Selection:

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. In vivo transplantation of MOLM-13 cells into NSG mice MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Negative Control:

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. In vivo transplantation of MOLM-13 cells into NSG mice MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..

    Article Title: A transcription factor addiction in leukemia imposed by the MLL promoter sequence
    Article Snippet: 2μg RNA was treated with DNaseI and reverse transcribed into cDNA using qScript cDNA SuperMix, then followed by RT-PCR with PCR mater mix containing SYBR green on ABI 7900HT fast real-time PCR machine. .. MOLM-13-Cas9 cells were first transduced with a Luciferase expressing cassette in Lenti-luciferase-P2A-Neo (Addgene #105621, Xu et al. 2018) vector, followed by G-418 (1mg/ml) selection and then viral transduction with LRG2.1-sgRNA-GFP vectors targeting the ZFP64 gene and Negative control (Neg1). ..



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: A membrane-associated MHC-I inhibitory axis for cancer immune evasion

    doi: 10.1016/j.cell.2023.07.016

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Final vectors were obtained by XhoI/EcoRI cloning of the 125 nt purified PCR products into recipient vectors. sgRNAs were designed and cloned into the LRG2.1-GFP sgRNA vector (Addgene #108098), or LRG2.1-TagBFP2 sgRNA vector (Addgene #124773), or LRCherry2.1 sgRNA vector (Addgene #108099) following the shRNA/sgRNA Cloning Protocol from the Broad Institute.

    Techniques: Control, Purification, Blocking Assay, Virus, Recombinant, Protease Inhibitor, Transfection, Immunoprecipitation, Activation Assay, Magnetic Beads, Reverse Transcription, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Double Knockout, shRNA, Real-time Polymerase Chain Reaction, Genome Wide, Plasmid Preparation, Software, Flow Cytometry